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A platform of BRET-FRET hybrid biosensors for optogenetics, chemical screening, and in vivo imaging.

Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada Y, Nagai T, Matsuda M
Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108652pCAGGS-6011nesAn ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
108653pCSIIbsr-6085xA tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
108654pCSIIbsr-6013xAn HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
108655pCAGGS-6017nesA PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
108656pCAGGS-6020nesA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)
108657pCAGGS-6015nesA S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Antibodies from Article