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Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair.

Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara S, Torii KU
Nat Chem Biol. 2018 Mar;14(3):299-305. doi: 10.1038/nchembio.2555. Epub 2018 Jan 22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108545pAN19-3xFLAG-TIR1-NOStCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator
108546pAN19-3xFLAG-ccvTIR1-NOStCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
108547pAN19-TIR1-GUS-NOStCloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator
108548pAN19-ccvTIR1-GUS-NOStCloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

Antibodies from Article