Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors.
Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD
Cell. 2018 Mar 8. pii: S0092-8674(18)30207-1. doi: 10.1016/j.cell.2018.02.033.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 109049 | pXR001: EF1a-CasRx-2A-EGFP | CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting |
| 109050 | pXR002: EF1a-dCasRx-2A-EGFP | Catalytically inactive dCasRx with 2A-EGFP |
| 109053 | pXR003: CasRx gRNA cloning backbone | hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning. |
| 109054 | pXR004: CasRx pre-gRNA cloning backbone | hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs |