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Construction and Use of a Recyclable Marker To Examine the Role of Major Facilitator Superfamily Protein Members in Candida glabrata Drug Resistance Phenotypes.

Vu BG, Moye-Rowley WS
mSphere. 2018 Mar 28;3(2). pii: mSphere00099-18. doi: 10.1128/mSphere.00099-18. eCollection 2018 Mar-Apr. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108363pBV65A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
108364pBV163Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.

Antibodies from Article