MIDAS: A Modular DNA Assembly System for Synthetic Biology.
van Dolleweerd CJ, Kessans SA, Van de Bittner KC, Bustamante LY, Bundela R, Scott B, Nicholson MJ, Parker EJ
ACS Synth Biol. 2018 Apr 20;7(4):1018-1029. doi: 10.1021/acssynbio.7b00363. Epub 2018 Apr 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108332 | pML1 | MIDAS Level-1 source vector for cloning of modules (synthetic biology parts) |
| 108333 | pML2(+)WF | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly. |
| 108334 | pML2(+)BF | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly. |
| 108335 | pML2(+)WR | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly. |
| 108336 | pML2(+)BR | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly. |
| 108337 | pML2(−)WF | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly. |
| 108338 | pML2(−)BF | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly. |
| 108339 | pML2(−)WR | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly. |
| 108340 | pML2(−)BR | MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly. |
| 108341 | pML3 | MIDAS Level-3 destination vector. Used for initiating Level-3 multigene assembly. |