Skip to main content
Addgene

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 with improved proof-reading enhances homology-directed repair.

Kato-Inui T, Takahashi G, Hsu S, Miyaoka Y
Nucleic Acids Res. 2018 May 18;46(9):4677-4688. doi: 10.1093/nar/gky264. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
108292pX459V2.0-eSpCas9(1.1)pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations
108293pX459V2.0-SpCas9-HF1pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations
108294pX459V2.0-HypaCas9pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations
108295pX459V2.0-SpCas9-HF4pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations
108296pX459V2.0+K848ApX459 V2.0 (Plasmid #62988) with the K848A mutation
108297pX459V2.0-eSpCas9(1.1)+K810ApX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations
108298pX459V2.0-eSpCas9(1.1)+K855ApX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations
108299pX459V2.0-eSpCas9(1.1)+K810A+K855ApX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations
108300pX459V2.0+N497A+Q695A+Q926ApX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutations
108301pX330-SpCas9-HF1pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations
108302pX330-HypaCas9pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

Antibodies from Article