Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene.
Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R
Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108245 | Fuw-dCas9-Tet1CD-P2A-BFP | Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP |
| 108246 | Fuw-dCas9-dead Tet1CD-P2A-BFP | Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP |
| 108247 | Fuw-AcrIIA4-P2A-GFP | Lentiviral vector to express AcrIIA4-P2A-GFP |
| 108248 | pgRNA-CGG | Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9 |