Artificial Self-Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity.
Haga T, Hirakawa H, Nagamune T
Biotechnol J. 2018 Dec;13(12):e1800088. doi: 10.1002/biot.201800088. Epub 2018 Aug 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108188 | pET15T+1X | PCNA1-PdX fusion protein |
| 108189 | pET15b+1(G108C)X | Expresses PCNA1(G108C)-PdX fusion protein in E. coli |
| 108191 | pET15b+R2(L171C)X | Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli |
| 108192 | pET15b+2(L171C)X | Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli |
| 108193 | pET28b+3(R112C/T180C)C | Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli |
| 108194 | pET28b+R3(R112C/T180C)C | Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli |
| 108195 | pET28b+R3(R112C/T180C) | Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli |
| 108196 | pET15b+C3(R112C/T180C)X | Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli |
| 108239 | pET15b+R2(L171C) | Expresses PdR-PCNA2(L171C) fusion protein in E. coli |