LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR
Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 108098 | LRG2.1 | Lentiviral expression plasmid of sgRNA with GFP |
| 108099 | LRCherry2.1 | Lentiviral expression plasmid of sgRNA with mCherry |
| 108100 | LentiV_Cas9_puro | Lentiviral expression plasmid of spCas9 with puromycin resistance gene |
| 108101 | LentiV_Neo | Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector) |
| 108103 | LentiV_Neo_SIK3 | Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene |
| 108104 | LentiV_Neo_SIK3_CR | Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene |
| 108106 | LentiV_Neo_SIK3_CR_K95M | Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance gene |
| 108107 | LentiV_Neo_SIK3_CR_T221A | Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene |
| 108108 | LentiV_Neo_SIK3_CR_T221E | Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene |
| 108109 | LentiV_Neo_SIK3_CR_T142Q | Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance gene |
| 108110 | LentiV_Neo_SIK3_T142Q | Lentiviral expression plasmid of human SIK3 cDNA (gatekeeper mutation) with neomycin resistance gene |
| 108111 | LentiV_Neo_LKB1 | Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene |
| 108112 | LentiV_Neo_LKB1_CR | Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene |
| 117725 | Human Kinase Domain-Focused CRISPR Knockout Library | gRNAs targeting the kinase domain of human protein kinases |