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Programmed hierarchical patterning of bacterial populations.

Boehm CR, Grant PK, Haseloff J
Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107945p1wtVT-lacIRatiometric reporter [PT7-RBSB0034, high copy number]
107946p4wtVT-lacIRatiometric reporter [PT7-RBSB0034, low copy number]
107947p1g3VT-lacIRatiometric reporter [PT7(-3G)-RBSB0034, high copy number]
107948p4g3VT-lacIRatiometric reporter [PT7(-3G)-RBSB0034, low copy number]
107949p1enVT-lacIRatiometric reporter [PT7-RBST7g10, high copy number]
107951p1eng3VT-lacIRatiometric reporter [PT7(-3G)-RBST7g10, high copy number]
107952p4eng3VT-lacIRatiometric reporter [PT7(-3G)-RBST7g10, low copy number]
107953p4g3VTRatiometric reporter [PT7(-3G)-RBSB0034, low copy number, LacI generator removed]
107954pTara:500*Arabinose-inducible T7*RNAP
107955pTSara*Arabinose-inducible split T7*RNAP
107956pTSaraSZArabinose-inducible split T7RNAP with SynZIP domains 17/18
107957pTSara*SZArabinose-inducible split T7*RNAP with SynZIP domains 17/18
107958p4g3RRFP reporter [PT7(-3G)-RBSB0034, low copy number]
107959pCRB DRT7VTPLux*500Controller plasmid [ca. 558.5 relative RBS strength at PLas81*]
107960pCRB DRT7VTPLux*500CFPCFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]
107961pCRB DRT7VTPLux*500YFPYFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]
107962pCRB DRT7VTPLux*250Controller plasmid [ca. 249.0 relative RBS strength at PLas81*]
107963pCRB DRT7VTPLux*100Controller plasmid [ca. 110.5 relative RBS strength at PLas81*]
107964pCRB DRT7VTPLux*50Controller plasmid [ca. 47.0 relative RBS strength at PLas81*]
107965pSB1C3 I0500 LuxIArabinose-inducible 3OC6HSL sender plasmid
107966pSB1C3 I0500 LasIArabinose-inducible 3OC12HSL sender plasmid

Antibodies from Article