Escherichia coli as a platform for the study of phosphoinositide biology.
Botero S, Chiaroni-Clarke R, Simon SM
Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. eCollection 2019 Mar.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107862 | p15aC-GFP | Control plasmid expressing GFP |
| 107863 | p15aC-1D | Bacterial expression of PIS |
| 107864 | p15aC-4D1D | Bacterial expression of PIS and PI4K |
| 107865 | p15aC-1D-5 | Bacterial expression of PIS and PI4P5K |
| 107866 | p15aC-4D1D-5 | Bacterial expression of PIS, PI4K and PI4P5K |
| 107867 | pClodAcytCh-PIP2PHGFP | Bacterial expression of Cherry and GFP-PLCδ-PH domain |
| 107870 | pClodANL-Cherry | Bacterial expression of cherry-NanoLuc |
| 107871 | pClodANL-FGF2 | Bacterial expression of FGF2-NanoLuc |
| 107872 | pClodANL-FGF2YE | Bacterial expression of FGF2phosphomimic-NanoLuc |
| 107873 | pClodANL-FGF2NBAA | Bacterial expression of FGF2 minimum export mutant-NanoLuc |
| 107874 | pClodANL-FGF4 | Bacterial expression of FGF4-NanoLuc |
| 113346 | pClodAcytCh-GFP-P4M | Bacterial expression of Cherry and GFP-SidM-P4M domain (2 copies) |
| 121050 | p15aC-3D1D-5 | Bacterial expression of PIS, PI4K and PI3P5K mutant E545K |