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Rewritable multi-event analog recording in bacterial and mammalian cells.

Tang W, Liu DR
Science. 2018 Feb 15. pii: science.aap8992. doi: 10.1126/science.aap8992. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107883pWT004aR1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
107884pWT004bR2 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
107885pWT018aR3 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
107886pWT018eR4 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
107887pWT004jR5 plasmid. Contains PBAD-EGFP-115-135insertion, T206G and is part of the CAMERA system for rewritable multi-event analog recording in bacteria
107888pWT009dR6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteria
107889pWT007aW1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1
107890pWT007gW1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2
107891pWT007fW1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3
107892pWT007hW1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c
107893pWT019aW1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1
107894pWT019bW1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2
107895pWT021aW2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0
107896pWT037aW2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1
107897pWT046aW2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4
107898pWT046bW2.3 plasmid. Contains PTetO-SD8-BE2, PRha-sgRNA4 and is part of CAMERA 2.3
107899pWT050aW2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4
107900pWT046eW2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5
107901pWT050dW2.5-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA6-(sgRNA4-C16T-C18T) and is part of CAMERA 2.5
107902pWT045cW2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6
107903pWT047cW2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7
107904pWT060bW2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5
107905pWT060eW2m.0-3, W2m.3-2 plasmid. Contains U6-sgRNA B (CCR5) and is part of the CAMERA2m system for cellular recording in mammalian cells
107906pWT060jW2m.0-4 plasmid. Contains U6-sgRNA C (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107907pWT060fW2m.1-2 plasmid. Contains U6-sgRNA D (CCR5) and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107908pWT063aW2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107909pWT062aW2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107910pWT062eW2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107911pWT062dW2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107912pWT065aW2m.3.1-1 plasmid. Contains (TCF-LEF)7-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells
107913pWT065cW2m.3.2-1 plasmid. Contains (TCF-LEF)7-BE3-P2A-Luc and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Antibodies from Article