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Temporally precise single-cell-resolution optogenetics.

Shemesh OA, Tanese D, Zampini V, Linghu C, Piatkevich K, Ronzitti E, Papagiakoumou E, Boyden ES, Emiliani V
Nat Neurosci. 2017 Dec;20(12):1796-1806. doi: 10.1038/s41593-017-0018-8. Epub 2017 Nov 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107708pAAV-hSynapsin- soCoChR-GFPA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter
107709pAAV-EF1alpha-soCoChR-GFPA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter
107710pAAV-CAG-soCoChR-GFPA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter
107711pAAV-CaMKII-soCoChR-GFPA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter
107712pAAV-hSynapsin-FLEX-soCoChR-GFPA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus
107713pAAV-hSyn-soCoChR-mCardinalA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter
107714pAAV-EF1alpha-soCoChR-tdTomatoA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Antibodies from Article