Spatiotemporal allele organization by allele-specific CRISPR live-cell imaging (SNP-CLING).
Maass PG, Barutcu AR, Shechner DM, Weiner CL, Mele M, Rinn JL
Nat Struct Mol Biol. 2018 Jan 8. pii: 10.1038/s41594-017-0015-3. doi: 10.1038/s41594-017-0015-3.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107605 | pUBC_HA_PP7-mCherry | Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag |
| 107606 | pUBC_V5_MS2-mVenus | Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag |
| 107607 | pUBC_hsPUM1-mCherry | Transient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoter |
| 107608 | pU6_T7_INT[6xminimal_Puf1] | Transient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoter |
| 107609 | pUBC_hsPUM1-iRFP670 | Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter |
| 111268 | pUBC_HA_PP7-mVenus | Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag |