Scot Wolfe Lab: Inducible Cas9-DNA-binding-domain fusions for precise genome editing
Scot Wolfe
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107298 | pCSD_N-SpCas9-1-573-FRB-1xNLS | Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells |
| 107299 | pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS | Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells |
| 107300 | pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS | Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells |
| 107301 | pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3 | Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells |
| 107302 | pCSD_SpCas9MT3-FKBP_SL | Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells |
| 107303 | pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS | Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells |
| 107304 | pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS | Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells |
| 107305 | pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL | Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells |
| 107306 | pCSD_FRB-TALETS3-3xFLAG-2xNLS | Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells |