Modification of C Terminus Provides New Insights into the Mechanism of alpha-Synuclein Aggregation.
Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107292 | 1a. pETM11-SUMO-SNCA-GFP | Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility |
| 107293 | s2. pT7-7-SNCA-5Asp | Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly |
| 107294 | t2. pT7-7-SNCA-5Lys | Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly |
| 107295 | r. pT7-7-SNCA-5Asn | Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly |
| 107296 | r-6. pT7-7-SNCA-2Asn | Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly |
| 107297 | r-22. pT7-7-SNCA-9Asn | Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly |