Microhomology-assisted scarless genome editing in human iPSCs.
Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107271 | CAG-Avr-HPRT-LEFT | Expresses TALEN (left) targeting HPRT exon3 |
| 107272 | CAG-Avr-HPRT-RIGHT | Expresses TALEN (right) targeting HPRT exon3 |
| 107273 | pX-EGFP-g1 | eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1) |
| 107274 | pX-APRT-sg2 | APRT sgRNA-2 and Cas9 expression vector |
| 107276 | p3-HPRT1-S104R-PdTK-mCh | HPRT1 c.312C>A Munich donor vector with unilateral microhomology |
| 107277 | p3-HPRT1-S104Rf-PdTK-mCh | HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology |
| 107279 | pbG-APRT-J-u32uni-PdTKmCh | APRT c.407T>C (APRT*J) donor vector with unilateral microhomology |
| 107280 | pAAVS1-PdTK-CAG-mCh-[uBglII] | puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry |
| 107281 | pCAG-eGFP-pA | donor vector backbone with constitutive eGFP |