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Spindle asymmetry drives non-Mendelian chromosome segregation.

Akera T, Chmatal L, Trimm E, Yang K, Aonbangkhen C, Chenoweth DM, Janke C, Schultz RM, Lampson MA
Science. 2017 Nov 3;358(6363):668-672. doi: 10.1126/science.aan0092. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107264mouse CENP-B-EGFPmouse CENP-B-EGFP in pIVT vector for in vitro transcription
1072656x Halo-EGFP- 2x PACT6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription
107266mCherry-eDHFRmCherry-eDHFR in pIVT vector for in vitro transcription
107267mCherry-eDHFR-Cdc42Q61LmCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

Antibodies from Article