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Extending roGFP Emission via Forster-Type Resonance Energy Transfer Relay Enables Simultaneous Dual Compartment Ratiometric Redox Imaging in Live Cells.

Norcross S, Trull KJ, Snaider J, Doan S, Tat K, Huang L, Tantama M
ACS Sens. 2017 Nov 22;2(11):1721-1729. doi: 10.1021/acssensors.7b00689. Epub 2017 Nov 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107218GW1-mito-roGFP1-mAppleMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.
107219GW1-mito-mRuby2-roGFP1Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.
107220GW1-mito-mCherry-roGFP2Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

Antibodies from Article