Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.
Penewit K, Holmes EA, McLean K, Ren M, Waalkes A, Salipante SJ
MBio. 2018 Feb 20;9(1). pii: mBio.00067-18. doi: 10.1128/mBio.00067-18.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107191 | pCN-EF2132tet | Expresses EF2132 recombinase for performing recombineering in S. aureus |
| 107192 | pCAS9counter | Expresses CAS9 and sgRNA for targeted counterselection in S. aureus |