Predictable and precise template-free CRISPR editing of pathogenic variants.

Shen MW, Arbab M, Hsu JY, Worstell D, Culbertson SJ, Krabbe O, Cassa CA, Liu DR, Gifford DK, Sherwood RI
Nature. 2018 Nov;563(7733):646-651. doi: 10.1038/s41586-018-0686-x. Epub 2018 Nov 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
107186	p2T-CAG-MCS-P2A-GFP-PuroR	Base plasmid for cloning gene duplication alleles with eGFP reporter.
107189	p2T-CAG-KKHSaCas9-BlastR	Confers constitutive expression of KKH SaCas9.
107190	p2T-CAG-SpCas9-BlastR	Confers constitutive expression of SpCas9