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Efficient proximity labeling in living cells and organisms with TurboID.

Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107167TurboID-V5_pRS415expresses V5-tagged TurboID in the yeast cytosol
107168miniTurbo-V5_pRS415expresses V5-tagged miniTurbo in the yeast cytosol
107169V5-TurboID-NES_pCDNA3expresses V5-tagged TurboID in the mammalian cytosol
107170V5-miniTurbo-NES_pCDNA3expresses V5-tagged miniTurbo in the mammalian cytosol
1071713xHA-TurboID-NLS_pCDNA3expresses 3xHA-tagged TurboID in the mammalian nucleus
1071723xHA-miniTurbo-NLS_pCDNA3expresses 3xHA-tagged miniTurbo in the mammalian nucleus
107173C1(1-29)-TurboID-V5_pCDNA3expresses V5-tagged TurboID on the mammalian ER membrane
107174C1(1-29)-miniTurbo-V5_pCDNA3expresses V5-tagged miniTurbo on the mammalian ER membrane
107175C1(1-29)-TurboID-V5_pLX304expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector
107176C1(1-29)-miniTurbo-V5_pLX304expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector
107177TurboID-His6_pET21aexpresses His6-tagged TurboID in bacteria
107178miniTurbo-His6_pET21aexpresses His6-tagged miniTurbo in bacteria
1182203xHA-TurboID_pAS31expresses 3xHA-tagged TurboID in C. elegans intestine
1182223xHA-miniTurbo_pAS32expresses 3xHA-tagged miniTurbo in C. elegans intestine
1182233xHA-BioID_pAS28expresses 3xHA-tagged BioID in C. elegans intestine

Antibodies from Article