Specific cyclic ADP-ribose phosphohydrolase obtained by mutagenic engineering of Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase.
Ribeiro JM, Canales J, Cabezas A, Rodrigues JR, Pinto RM, Lopez-Villamizar I, Costas MJ, Cameselle JC
Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.1038/s41598-017-18393-9.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107163 | pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM | Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme |