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Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.

Gassaway BM, Cardone RL, Padyana AK, Petersen MC, Judd ET, Hayes S, Tong S, Barber KW, Apostolidi M, Abulizi A, Sheetz JB, Kshitiz, Aerni HR, Gross S, Kung C, Samuel VT, Shulman GI, Kibbey RG, Rinehart J
Cell Rep. 2019 Dec 10;29(11):3394-3404.e9. doi: 10.1016/j.celrep.2019.11.009. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107155MBP-rPKL S12TAGMBP-rPKL construct for production and purification of rat PKL pS12
107156MBP-rPKL WTMBP-rPKL WT for production and purification of rat PKL WT
107157MBP-rPKL S113TAGMBP-rPKL S113TAG for production and purification of rat PKL pS113
107158MBP-rPKM2 WTMBP-rPKM2 WT for production and purification of rat PKM2 WT
107159Flag-hPKL WTFor mammalian expression of Flag-hPKL WT
107160Flag-hPKL S113AFor mammalian expression of Flag-hPKL S113A
107161Flag-hPKL S113DFor mammalian expression of Flag-hPKL S113D

Antibodies from Article