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Ultrahigh specificity in a network of computationally designed protein-interaction pairs.

Netzer R, Listov D, Lipsh R, Dym O, Albeck S, Knop O, Kleanthous C, Fleishman SJ
Nat Commun. 2018 Dec 11;9(1):5286. doi: 10.1038/s41467-018-07722-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
107108pET21d-colEdes1/Imdes1plasmid for expression of computationally designed colicin endonuclease and immunity pair, des1
107109pET21d-colEdes2/Imdes2plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2
107110pET21d-colEdes3/Imdes3plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3
107111pET21d-colEdes3.5/Imdes3.5plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5
107112pET21d-colEdes4/Imdes4plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4
107113pET21d-colEdes5/Imdes5plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5
107114pET21d-colEdes6/Imdes6plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6
107115pET21d-colEdes7/Imdes7plasmid for expression of computationally designed colicin endonuclease and immunity pair, des7
107116pET21d-colEdes8/Imdes8plasmid for expression of computationally designed colicin endonuclease and immunity pair, des8
107117pET21d-colEdes9/Imdes9plasmid for expression of computationally designed colicin endonuclease and immunity pair, des9
107118pET21d-colEdes10/Imdes10plasmid for expression of computationally designed colicin endonuclease and immunity pair, des10
107119pET21d-colEdes11/Imdes11plasmid for expression of computationally designed colicin endonuclease and immunity pair, des11
107120pET21d-colEdes12/Imdes12plasmid for expression of computationally designed colicin endonuclease and immunity pair, des12
107121pET21d-colEdes13/Imdes13plasmid for expression of computationally designed colicin endonuclease and immunity pair, des13
107122pET21d-colEdes14/Imdes14plasmid for expression of computationally designed colicin endonuclease and immunity pair, des14
107123pET21d-colEdes15/Imdes15plasmid for expression of computationally designed colicin endonuclease and immunity pair, des15
107124pET21d-colEdes16/Imdes16plasmid for expression of computationally designed colicin endonuclease and immunity pair, des16
107125pET21d-colEdes17/Imdes17plasmid for expression of computationally designed colicin endonuclease and immunity pair, des17
107126pET21d-colEdes18/Imdes18plasmid for expression of computationally designed colicin endonuclease and immunity pair, des18
107127pET21d-colEwt2/Imwt2plasmid for expression of WT colicin endonuclease and immunity pair 2

Antibodies from Article