Three proliferating cell nuclear antigen homologues from Metallosphaera sedula form a head-to-tail heterotrimer.
Iwata F, Hirakawa H, Nagamune T
Sci Rep. 2016 May 27;6:26588. doi: 10.1038/srep26588.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107063 | pET15T-MsePCNA1 | Expresses M. sedula PCNA1 in E. coli |
| 107064 | pET15T-MsePCNA2 | Expresses M. sedula PCNA2 in E. coli |
| 107065 | pET15T-MsePCNA3 | Expresses M. sedula PCNA3 in E. coli |
| 107069 | pMAL-SsoPCNA3 | Expresses S. solfataricus PCNA3 fused to MBP in E. coli |
| 107071 | pET28b-MseLig1 | Expresses M. sedula DNA Ligase 1 in E. coli |
| 107072 | pET15b-YC | Host vector to construct a YPer or CyPet fusion protein |
| 107073 | pMD-MsePCNA1 | M. sedula PCNA1 |
| 107074 | pMD-MsePCNA2 | M. sedula PCNA2 |
| 107075 | pMD-MsePCNA3 | M. sedula PCNA3 |
| 107076 | pMD-SsoPCNA1 | S. solfataricus PCNA1 |
| 107077 | pMD-SsoPCNA2 | S. solfataricus PCNA2 |
| 107078 | pMD-SsoPCNA3 | S. solfataricus PCNA3 |
| 107079 | pET15b-YM1 | M. sedula PCNA1 fused at the C-terminus of YPet |
| 107080 | pET15b-YM2 | M. sedula PCNA2 fused at the C-terminus of YPet |
| 107081 | pET15b-YM3 | M. sedula PCNA3 fused at the C-terminus of YPet |
| 107082 | pET15b-YS1 | S. solfataricus PCNA1 fused at the C-terminus of YPet |
| 107084 | pET15b-YS3 | S. solfataricus PCNA3 fused at the C-terminus of YPet |
| 107085 | pET15b-M1C | M. sedula PCNA1 fused at the N-terminus of CyPet |
| 107086 | pET15b-M2C | M. sedula PCNA2 fused at the N-terminus of CyPet |
| 107087 | pET15b-M3C | M. sedula PCNA3 fused at the N-terminus of CyPet |
| 107088 | pET15b-S1C | S. solfataricus PCNA1 fused at the N-terminus of CyPet |
| 107090 | pET15b-S3C | S. solfataricus PCNA3 fused at the N-terminus of CyPet |