Alex Hewitt Lab: Alex Hewitt Lab plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107023 | pX552-GFP | pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter. |
| 107024 | pX551-CMV-SpCas9 | AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab |
| 107031 | pX551-miniCMV-SpCas9 | AAV plasmid expressing Cas9 under control of miniCMV promoter |
| 107032 | pX551-miniCMV-SaCas9 | AAV plasmid expressing SaCas9 under control of miniCMV promoter |
| 107033 | pX551-miniCMV-CjCas9 | AAV plasmid expressing CjCas9 under control of miniCMV promoter |
| 107034 | pX551-miniCMV-AsCpf1 | AAV plasmid expressing AsCpf1 under control of miniCMV promoter |
| 107035 | pX551-CMV-CjCas9 | AAV plasmid expressing CjCas9 under control of CMV promoter |
| 107049 | pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA | All-in-one vectors expressing both SaCas9 and LacZ sgRNA |
| 107050 | pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA | All-in-one vectors expressing both SaCas9 and YFP sgRNA |
| 107055 | pX601 miniCMV-SaCas9-SpA-sgRNA scaffold | Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9) |