Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish.

Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q
Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
107590	Tol2-lyzC-mito-Dendra2	Expresses mitochondria localized Dendra2 in zebrafish neutrophils
107591	p5E-lyzC	An entry vector with a lyzC promoter
107592	pME-cas9-2A-mCherry	An entry vector with cas9 -2A-mCherry
107593	p3E-U6a	An entry vector with U6a promoter driving guide RNA expression
107594	pCS2-sod1	Contains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
107598	p3E-U6a-polg guide	An entry vector with U6a promoter driving polg guide RNA expression
107599	p3E-U6a-U6c	An entry vector with U6a and U6c promoter driving guide RNAs expression
107600	p3E-U6a-U6c-rac2 guide	An entry vector with U6a and U6c promoter driving rac2 guide RNAs expression
107601	p3E-U6a-U6c-ndufs2 guide	An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression
107602	p3E-U6a-U6c-uqcrc1 guide	An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression
107603	p3E-U6a-U6c-sod1 guide	An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression
107604	p3E-U6a-U6c-sod2 guide	An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression