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Applications of Lgr5-Positive Cochlear Progenitors (LCPs) to the Study of Hair Cell Differentiation.

Lenz DR, Gunewardene N, Abdul-Aziz DE, Wang Q, Gibson TM, Edge ASB
Front Cell Dev Biol. 2019 Feb 19;7:14. doi: 10.3389/fcell.2019.00014. eCollection 2019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
106948pLV U6-sgRNA UbC-GFPsgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker
106949pLV U6-sgRNA UbC-tdTomatosgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker
106950pLV sgRNA-Notch1 #1 GFPLentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.
106951pLV sgRNA-Notch1 #2 GFPLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.
106952pLV sgRNA-Notch1 #3 GFPLentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.
106953pLV sgRNA-Hic1 GFPLentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Antibodies from Article