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Gravin regulates centrosome function through PLK1.

Colicino EG, Garrastegui AM, Freshour J, Santra P, Post DE, Kotula L, Hehnly H
Mol Biol Cell. 2017 Dec 27. pii: mbc.E17-08-0524. doi: 10.1091/mbc.E17-08-0524. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
106905(PD-S608A)FLAG-Cep215-FLExpresses murine non-phosphorylatable Cep215 at S608
106906(PM-S608E)FLAG-Cep215-FLExpresses murine phospho-mimetic CEP215 at S608
106907PLK1-cjun-FRET-PACT biosensorcentrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)
106908pBABE-SSeCKsfull-length murine SSeCKs/AKAP12/Gravin in retroviral vector
107142pBABE-SSeCKs T751Afull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

Antibodies from Article