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Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.

Zhang Q, Huang H, Lu Q, Wu R, Chung CI, Zhang SQ, Torra J, Schepis A, Coughlin SR, Kornberg TB, Shu X
Mol Cell. 2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 10.1016/j.molcel.2017.12.008. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
106919pcDNA3-Frb-EGFP-HOTag6Express Frb-EGFP-HOTag6 fusion in mammalian cells.
106920pcDNA3-PKA-SPARKExpress PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.
106921pcDNA3-ERK-SPARKExpress ERK-SPARK, an activity reporter of ERK in mammalian cells.
106922pUASattB-PKA-SPARKExpress PKA-SPARK, an activity reporter of protein kinase A in Drosophila
106923pUASattB-ERK-SPARKExpress ERK-SPARK, an activity reporter of ERK in Drosophila
106924pcDNA3-FKBP-EGFP-HOTag3Expresses FKBP-EGFP-HOTag3 in mammalian cells

Antibodies from Article