Development of new versatile plasmid-based systems for lambdaRed-mediated Escherichia coli genome engineering.

Bubnov DM, Yuzbashev TV, Vybornaya TV, Netrusov AI, Sineoky SP
J Microbiol Methods. 2018 Jun 7. pii: S0167-7012(18)30368-3. doi: 10.1016/j.mimet.2018.06.001. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
106483	pDL17	pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome
106901	pDL14	pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
106902	pQE30del	pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.