A newly characterized vacuolar serine carboxypeptidase, Atg42/Ybr139w, is required for normal vacuole function and the terminal steps of autophagy in the yeast Saccharomyces cerevisiae.
Parzych KR, Ariosa A, Mari M, Klionsky DJ
Mol Biol Cell. 2018 May 1;29(9):1089-1099. doi: 10.1091/mbc.E17-08-0516. Epub 2018 Mar 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106461 | pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t) | Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells |
| 106462 | pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t] | Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells |
| 106464 | pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t) | Expresses Prc1 with a C-terminal GFP tag in yeast cells |
| 106465 | pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t) | Expresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cells |
| 106466 | pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t] | Expresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cells |
| 106467 | pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t] | Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells |
| 106468 | pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t] | Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells |
| 106469 | pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t] | Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells |
| 106470 | pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t) | Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells |
| 106471 | pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t) | Expresses Prc1 with a C-terminal PA tag in yeast cells |
| 106472 | pKP112 (pRS406-GFP-ADH1t) | Vector for creating C-terminal GFP fusion proteins in yeast. |