Genetic tools for reliable gene expression and recombineering in Pseudomonas putida.
Cook TB, Rand JM, Nurani W, Courtney DK, Liu SA, Pfleger BF
J Ind Microbiol Biotechnol. 2018 Jan 3. pii: 10.1007/s10295-017-2001-5. doi: 10.1007/s10295-017-2001-5.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106382 | pBBR1k-GFPuv | IPTG-inducible trc promoter expressing gfpuv on BBR1 origin |
| 106383 | pBBR2k-GFPuv | Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin |
| 106384 | pBBR6k-GFPuv | IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin |
| 106385 | pBBR8k-GFPuv | L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin |
| 106386 | pBBR1k-con-GFPuv | Constitutive trc promoter expressing gfpuv on BBR1 origin |
| 106387 | pBBR6k-con-GFPuv | Constitutive LlacO-1 promoter expressing gfpuv on BBR1 origin |
| 106388 | pBBR1k-J23101-GFPuv | pBBR1k-GFPuv with Anderson promoter J23101 expressing lacI |
| 106389 | pBBR1k-J23107-GFPuv | pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI |
| 106390 | pBBR6k-J23101-GFPuv | pBBR6k-GFPuv with Anderson promoter J23101 expressing lacI |
| 106391 | pBBR6k-J23107-GFPuv | pBBR6k-GFPuv with Anderson promoter J23107 expressing lacI |
| 106392 | pBBR6k-J23109-GFPuv | pBBR6k-GFPuv with Anderson promoter J23109 expressing lacI |
| 106393 | pBb(B5)1k-GFPuv | IPTG-inducible trc promoter expressing gfpuv on BBR1-B5 origin |
| 106394 | pBb(RK2)1k-GFPuv | IPTG-inducible trc promoter expressing gfpuv on RK2 origin |
| 106395 | pBb(RSF1010)1k-GFPuv | IPTG-inducible trc promoter expressing gfpuv on RSF1010 origin |
| 106397 | pgRNAtet-lvaA | Guide RNA plasmid targeting lvaA on BBR1-UP origin |
| 110140 | pBb(B5)8k-sfGFP | IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin |
| 110141 | pRK2-AraE | Constitutive promoter expressing araE on RK2 origin |
| 110142 | pJOE_pvdJ | Suicide plasmid with repair template for knockout in P. putida |