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Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells.

Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, Beisel CL, Barrangou R, Gersbach CA
Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 10.1038/s41587-019-0235-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
106270pcDNA-Flag-EcoCas8eEncodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
106271pcDNA-Flag-EcoCse2Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
106272pcDNA-Flag-EcoCas7Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
106273pcDNA-Flag-EcoCas5Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
106274pcDNA-Flag-EcoCas6Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
106275pcDNA-CasE-p300-FlagEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
106276pAPcrRNA_EcoE.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter
126481pEcoCascade-IL1RNcrRNAEncodes E.coli CRISPR Type I-E crRNA targeting IL1RN
126482pcDNA-Flag-LmoCas8b2Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
126483pcDNA-Flag-LmoCas7Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
126484pcDNA-Flag-LmoCas5Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
126485pcDNA-Flag-LmoCas6Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
126486pcDNA-LmoCas6-p300Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
126487pLmoCascade-IL1RNcrRNAEncodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN
126488pLenti-LmoCas8b2Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter
126489pLenti-LmoCas7Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter
126490pLenti-LmoCas5Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter
126491pLenti-LmoCas6Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter
126492pLenti-LmoCas6-KRAB-2a-BlastREncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter
126493pLenti-LmoCascade-IL1RNcrRNAEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN
126494pLenti-LmoCascade-HBE1crRNAEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1
126501pAPcrRNA_LmoL.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter

Antibodies from Article