The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells.
Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA
Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104094 | pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA | Expresses TREAT reporter in mammalian cells |
| 104095 | pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA | Expresses TREAT-siRNA reporter in mammalian cells |
| 104096 | pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA | Expresses TREAT-ARE reporter in mammalian cells |
| 104097 | pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA | Expresses TREAT-PK co-localization control in mammalian cells |
| 104098 | phage UbiC NLS HA stdMCP Halo | Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs. |
| 104099 | phage UbiC NLS HA stdPCP stdGFP | Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs. |
| 104140 | pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA | Expresses TREAT-5'TOP reporter in mammalian cells |