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Dual gene activation and knockout screen reveals directional dependencies in genetic networks.

Boettcher M, Tian R, Blau JA, Markegard E, Wagner RT, Wu D, Mo X, Biton A, Zaitlen N, Fu H, McCormick F, Kampmann M, McManus MT
Nat Biotechnol. 2018 Jan 15. pii: nbt.4062. doi: 10.1038/nbt.4062. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105996sgLentisingle sgRNA expression vector
105997sgLenti-orthogonalDual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector
105998pMB-SaCas9SaCas9 expression vector

Antibodies from Article