An efficient method to generate conditional knockout cell lines for essential genes by combination of auxin-inducible degron tag and CRISPR/Cas9.
Nishimura K, Fukagawa T
Chromosome Res. 2017 Oct;25(3-4):253-260. doi: 10.1007/s10577-017-9559-7. Epub 2017 Jun 6.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105985 | pAID 1.1 N-T2A-Bsr | To make an auxin inducible degron mutant |
| 105986 | pAID 1.1C-T2A-Bsr | To make an auxin inducible degron mutant |