Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble.
Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105924 | pMK231 (AAVS1 CMV-MCS-PURO) | Donor for integration at the human AAVS1 locus |
| 105927 | hROSA26 CRISPR-pX330 | hROSA26 targeting CRISPR plasmid |
| 114686 | pTK511 | Express Mem-BFP-iLID |
| 114687 | pTK372 | sgRNA targeting NuMA's C-terminal locus |
| 114688 | pTK421 | RFP-Nano tagging at NuMA's C-terminal locus |
| 114689 | pTK308 | sgRNA targeting DHC's C-terminal locus |
| 114690 | pTK565 | SNAP-tagging at DHC's C-terminal locus |
| 114691 | pTK525 | sgRNA for p150's C-terminal locus |
| 114692 | pTK587 | SNAP-tagging at p150's C-terminal locus |
| 114693 | pTK371 | sgRNA for DHC's N-terminal locus |
| 114694 | pTK426 | Nano-mCherry tagging at DHC's N-terminal locus |
| 114695 | pTK583 | SNAP-tagging at NuMA's C-terminal locus |
| 114696 | pTK600 | SNAP-tagging at DHC's C-terminal locus (BSD) |
| 114697 | pTK630 | Express RFP-Nano from Rosa 26 locus |
| 114698 | pTK715 | Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus |
| 114699 | pTK713 | Express NuMA(1-2115ΔNLS)-RFP-Nano from Rosa 26 locus |
| 114700 | pTK652 | Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus |
| 114701 | pTK631 | Express NuMA(1-705)-RFP-Nano from Rosa 26 locus |
| 114702 | pTK660 | Express NuMA(1-705)4A-RFP-Nano from Rosa 26 locus |
| 114703 | pTK643 | Express NuMA(214-705)-RFP-Nano from Rosa 26 locus |
| 114704 | pTK664 | Express NuMA(1-505)-RFP-Nano from Rosa 26 locus |
| 114705 | pTK641 | Express NuMA(1-413)-RFP-Nano from Rosa 26 locus |
| 114706 | pTK642 | Express NuMA(1-213)-RFP-Nano from Rosa 26 locus |
| 114707 | pTK678 | Express NuMA(1-1895)-RFP-Nano from Rosa 26 locus |
| 114708 | pTK711 | Express NuMA(1-1985)-RFP-Nano from Rosa 26 locus |
| 114710 | pTK729 | Express NuMA(1-705+1700-2115ΔNLS)-RFP-Nano from Rosa 26 locus |
| 114711 | pTK645 | Express NuMA(1700-2115)-RFP-Nano from Rosa 26 locus |
| 114712 | pTK746 | Express NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locus |
| 114713 | pTK388 | mAID-mClover-3FLAG tagging at DHC's C-terminal locus |
| 114714 | pTK398 | mAID-mClover-3FLAG tagging at NuMA's C-terminal locus |
| 114715 | pTK473 | sgRNA for LGN's N-terminal locus |
| 114716 | pTK586 | SNAP-tagging at LGN's N-terminal locus (BSD) |
| 114717 | pTK503 | Express mCherry-NuMA(WT) from Rosa 26 locus |
| 114718 | pTK750 | Express mCherry-NuMA(5A-3) from Rosa 26 locus |