A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria.
Chang HJ, Mayonove P, Zavala A, De Visch A, Minard P, Cohen-Gonsaud M, Bonnet J
ACS Synth Biol. 2017 Oct 30. doi: 10.1021/acssynbio.7b00266.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107237 | pHJ1_Full length LexA | sfGFP expression repressed by transcriptional factor LexA |
| 108240 | pHJ2: LexA DBD | LexA DNA binding domain |
| 108241 | pHJ3: LexA-VHH-Control | LexA DNA binding domain fusing with anti-RNase A VHH antibody |
| 108242 | pHJ4: LexA-VHH-Caffeine | LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression. |
| 108243 | pHJ8: CadC-VHH-Control | membrane bound transcription activator CadC fusing with anti-RNase A antibody |
| 108244 | pHJ12: CadC-VHH-Caffeine (No linker) | Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression |