Transcription Factor-Mediated Differentiation of Human iPSCs into Neurons.
Fernandopulle MS, Prestil R, Grunseich C, Wang C, Gan L, Ward ME
Curr Protoc Cell Biol. 2018 Jun;79(1):e51. doi: 10.1002/cpcb.51. Epub 2018 May 18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105840 | pUCM-AAVS1-TO-hNGN2 | Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron |
| 105841 | pUCM-CLYBL-hNIL | Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron |
| 105842 | CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL)) | Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment |