Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Szczesny RJ, Kowalska K, Klosowska-Kosicka K, Chlebowski A, Owczarek EP, Warkocki Z, Kulinski TM, Adamska D, Affek K, Jedroszkowiak A, Kotrys AV, Tomecki R, Krawczyk PS, Borowski LS, Dziembowski A
PLoS One. 2018 Mar 28;13(3):e0194887. doi: 10.1371/journal.pone.0194887. eCollection 2018. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
105814	pKK-RNAi-nucCHERRYmiR-TEV-EGFP	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
105813	pKK-RNAi-nucCHERRYmiR-TEV-FLAG	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
105812	pKK-RNAi-nucEGFPmiR-TEV-mCherry	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.
105811	pKK-RNAi-nucEGFPmiR-TEV-FLAG	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
105810	pKK-RNAi-nucCHERRYmiR-EGFP-TEV	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
105809	pKK-RNAi-nucCHERRYmiR-FLAG-TEV	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.
105808	pKK-RNAi-nucEGFPmiR-mCherry-TEV	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.
105807	pKK-RNAi-nucEGFPmiR-FLAG-TEV	Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.
105806	pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber	Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
105805	pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus	Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.