FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling.
Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC
Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 85845 | pDONOR-SNCAe2-WT | Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP |
| 85847 | pDONOR-SNCAe3-WT | Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato |
| 85848 | pDONOR-SNCAe3-A53T | Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP |
| 86154 | pDONOR-PARK6e5-I368N | Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato |
| 100603 | pDONOR-tagBFP-PSM-EGFP | Donor scaffold with EGFP positive selection module, and tagBFP negative selection module |
| 100604 | pDONOR-tagBFP-PSM-dTOMATO | Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module |