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Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination.

Juszkiewicz S, Hegde RS
Mol Cell. 2017 Feb 16;65(4):743-750.e4. doi: 10.1016/j.molcel.2016.11.039. Epub 2017 Jan 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105686pmGFP-P2A-K0-P2A-RFPDouble fluorescent stalling reporter K0
105687pmGFP-P2A-K(AAA)12-P2A-RFPDouble fluorescent stalling reporter K(AAA)12
105688pmGFP-P2A-K(AAA)20-P2A-RFPDouble fluorescent stalling reporter K(AAA)20
105689pmGFP-P2A-K(AAG)20-P2A-RFPDouble fluorescent stalling reporter K(AAG)20
105690pcDNA3.1-ZNF598-TEV-3xFLAGExpresses C terminally 3xFLAG tagged ZNF598 protein

Antibodies from Article