Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems.

Lowder LG, Zhou J, Zhang Y, Malzahn A, Zhong Z, Hsieh TF, Voytas DF, Zhang Y, Qi Y
Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
100044	pYPQ131D2.0	Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
99907	pYPQ173	Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
99906	pYPQ141D2.0	Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
99905	pYPQ141C2.0	Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
99897	pYPQ141B2.0	Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
99896	pYPQ141A2.0	Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter
99895	pYPQ140	Empty vector with attL5-attL2 recombination sites for Gateway Cloning
99894	pYPQ133D2.0	Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter
99893	pYPQ133C2.0	Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter
99892	pYPQ133B2.0	Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter