Robust transcriptional activation in plants using multiplexed CRISPR-Act2.0 and mTALE-Act systems.
Lowder LG, Zhou J, Zhang Y, Malzahn A, Zhong Z, Hsieh TF, Voytas DF, Zhang Y, Qi Y
Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 10.1016/j.molp.2017.11.010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 99325 | pYPQ121 | Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence |
| 99882 | pFUS_A8 | Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats. |
| 99883 | pYPQ127B | Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoter |
| 99884 | pYPQ131A2.0 | Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter |
| 99885 | pYPQ131B2.0 | Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 99886 | pYPQ131C2.0 | Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter |
| 99887 | pYPQ132A2.0 | Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter |
| 99888 | pYPQ132B2.0 | Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 99889 | pYPQ132C2.0 | Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter |
| 99890 | pYPQ132D2.0 | Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter |
| 99891 | pYPQ133A2.0 | Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter |
| 99892 | pYPQ133B2.0 | Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 99893 | pYPQ133C2.0 | Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter |
| 99894 | pYPQ133D2.0 | Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter |
| 99895 | pYPQ140 | Empty vector with attL5-attL2 recombination sites for Gateway Cloning |
| 99896 | pYPQ141A2.0 | Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter |
| 99897 | pYPQ141B2.0 | Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter |
| 99905 | pYPQ141C2.0 | Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter |
| 99906 | pYPQ141D2.0 | Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter |
| 99907 | pYPQ173 | Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence |
| 100044 | pYPQ131D2.0 | Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter |