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Thanos Halazonetis Lab: Expression plasmids for production of Rev7 and Champ1 proteins

Thanos Halazonetis
Unpublished

Plasmids from Article

ID Plasmid Purpose
105630pT5T_Rev7_1-211_SA112_KVK_YAKExpresses mouse monomeric Rev7 protein with mutations abolishing binding to Champ1 and Rev3
105634pT5T_Rev7_1-211_SA112_KVKExpresses mouse monomeric Rev7 protein
105635pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
105637pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
105638pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid
105639pIRESN2_GFP_YTA_Champ1_328-354Expresses fragment (amnio acids 328-354) of human Champ1 protein tagged with GFP
105672pCDZ2FE_Rev3_1873-2025Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein
105673pCDZ2FE_Rev3_1873-2025_4PAExpresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding
105674pCDZ2FE_Pogz_1-1410Expresses Flag-tagged full-length human POGZ protein
105675pCDZ2FE_Champ1_1-812Expresses Flag-tagged full-length human Champ1 protein
105676pCDZ2FE_Champ1_1-812_2PAExpresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding

Antibodies from Article