SnoopLigase Catalyzes Peptide-Peptide Locking and Enables Solid-Phase Conjugate Isolation.
Buldun CM, Jean JX, Bedford MR, Howarth M
J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 10.1021/jacs.7b13237. Epub 2018 Feb 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 105626 | pET28a-AviTag-SnoopLigase | Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation |
| 105627 | pET28a-HaloTag7-SnoopLigase | Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7 |
| 105628 | pET28a-SnoopTagJr-MBP | Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase |
| 105629 | pET28a-SUMO-DogTag | Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr |