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A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.

Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR
Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 10.1016/j.ccell.2017.12.002. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
105559LEP-shTAF12.364retrovirally express of TAF12 shRNA with puro resistance and GFP marker
105560LEP-shTAF12.376retrovirally express TAF12 shRNA with puro resistance and GFP marker
105561LEP-shTAF2.1635retrovirally express TAF2 shRNA with puro resistance and GFP marker
105562LEP-shTAF2.4392retrovirally express TAF2 shRNA with puro resistance and GFP marker
105563LEP-shTAF4.2818retrovirally express TAF4 shRNA with puro resistance and GFP marker
105564LEP-shTAF4.3818retrovirally express TAF4 shRNA with puro resistance and GFP marker
105565LEP-shMLL-AF9.643retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker
105566LEP-shMLL-AF9.1039retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker
105567LEP-shTADA1.1226retrovirally express TADA1 shRNA with puro resistance and GFP marker
105568LEP-shTADA1.1580retrovirally express TADA1 shRNA with puro resistance and GFP marker
105572TRIN-E-shTAF12.364Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance
105573TRIN-E-shTAF12.376Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance
105574TRIN-shMYB.2572Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance
105575TRIN-shMYB.2652Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance
105577MLSE-shTAF12.364retrovirally express TAF12 shRNA with GFP marker
105578MLSE-shTAF12.376retrovirally express TAF12 shRNA with GFP marker
105579LENC-shTAF12.364retrovirally express TAF12 shRNA with Neo resistance and mCherry marker
105580LEP-shRENretrovirally express control shRNA with puro resistance and GFP marker
105583MLSE-shRENretrovirally express control shRNA with GFP marker
105584MLSE-shRPA3.457retrovirally express RPA3 shRNA with GFP marker
105585TRIN-E-shRENDox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance
105586TRIN-shRENDox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistance
105587LRG-2.1T-neo-sgROSAlentivirally express gRNA targeting ROSA26 locus with GFP marker and neo resistance
105588LRG-neo-sgTAF12(mouse)#4.1lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance
105590LRG-2.1T-neo-sgTAF12(human)#4.4lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance
105591LRG-2.1T-neo-sgMYB(human)#4.9lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance
105592LRG-2.1T-neo-sgMYB(human)#5.2lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance
105593MSCV-PIG-N3FLAG-TAF12retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance
105594MSCV-PIG-empty empty control vector with GFP marker and puro resistance
105595MSCV-PIG-N3FLAG-TAF12(50-130)retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance
105596MSCV-puro-N3FLAG-MYB-FLretrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance
105597MSCV-puro-N3FLAG-MYB-del(TAD)retrovirally express mouse MYB-del(TAD) with 3*FLAG tag at N terminal and puro resistance
105609PCDNA3-N3FLAG-MYB-FLtransient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells
105610PCDNA3-N3FLAG-MYB-DBD(1-200)transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells
105611PCDNA3-N3FLAG-MYB-TAD(251-327)transient express MYB TAD fragment with 3*FLAG tag at N terminal in HEK 293T cells
105612PCDNA3-N3FLAG-MYB-(1-327)transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells
105613PCDNA3-N3HA-TAF12-HFD(50-130)transient express TAF12 HFD with 3* HA tag at N terminal in HEK 293T cells
105614PCDNA3-TAF4(827-909)transient express TAF4 HFD in HEK 293T cells
105615PCDNA3-N3FLAG-TAF12-HFD(50-130)transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells
105616MSCV-PIG-TAF4(827-909)retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance
105617MSCV-PIG-FLAG-TAF4(827-909)retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,
105618pGEX-4T1-TAF12(50-130)bacterially express GST tagged TAF12 HFD
105619pGEX-4T1-MYB-TAD(251-327)bacterially express GST tagged MYB TAD
105620pET28a-TAF4(827-909)bacterially express TAF4 HFD
105621Lenti-luciferase-P2A-Neo3rd generation lentiviral plasmid for expression of luciferase with Neo resistance
105984PCDNA3-TADA1-HFD(140-230)transient express TADA1-HFD fragment in HEK 293T cells
105987LRG-2.1T-sgTAF12(human)#4.5 lentivirally express gRNA targeting human TAF12 HFD with GFP marker
105990MSCV-PIG-N3FLAG-TAF10retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance
106380TtGNN-GFP-emptydox inducible expression of GFP with neo resistance
106381TtGNN-TAF4-HFDdox inducible expression of GFP fused TAF4 HFD (827-909) with neo resistance

Antibodies from Article