Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers.
Carleton JB, Berrett KC, Gertz J
Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106399 | SID4x-dCas9-KRAB | Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain |
| 106404 | pGL3-U6-sgRNA-PGK-puromycin-AflII | Empty guide RNA cloning vector with an AflII site added from Addgene 41824 |