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Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration.

Hsu AY, Wang D, Liu S, Lu J, Syahirah R, Bennin DA, Huttenlocher A, Umulis DM, Wan J, Deng Q
Proc Natl Acad Sci U S A. 2019 Aug 26. pii: 1905221116. doi: 10.1073/pnas.1905221116. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
97102Tol2-lyzC-microRNA 7a-2-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97103Tol2-lyzC-microRNA Let 7-2-dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97104Tol2-lyzC-microRNA 19c-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97105Tol2-lyzC-microRNA 22b-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97106Tol2-lyzC-microRNA 24-1-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97107Tol2-lyzC-microRNA 31-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97108Tol2-lyzC-microRNA 96--dendra2overexpression of microRNA and Dendra2 in Zebrafish
97109Tol2-lyzC-microRNA 99-1-Dendra2overexpression of microRNA and Dendra2 in Zebrafish
97110Tol2-lyzC-microRNA 101a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97111Tol2-lyzC-microRNA 107b-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97112Tol2-lyzC-microRNA 141-dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97113Tol2-lyzC-microRNA 146a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97114Tol2-lyzC-microRNA 148-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97115Tol2-lyzC-microRNA 182-dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97116Tol2-lyzC-microRNA 184-1-dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97117Tol2-lyzC-microRNA 187-dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97118Tol2-lyzC-microRNA 190a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97119Tol2-lyzC-microRNA 199-3-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97120Tol2-lyzC-microRNA 200b-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97121Tol2-lyzC-microRNA 203b-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97122Tol2-lyzC-microRNA 212-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97123Tol2-lyzC-microRNA 214-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97124Tol2-lyzC-microRNA 216a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97125Tol2-lyzC-microRNA 218a-2-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97126Tol2-lyzC-microRNA 301a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97127Tol2-lyzC-microRNA 375-1-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97128Tol2-lyzC-microRNA 455a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97129Tol2-lyzC-microRNA 457a-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97131Tol2-lyzC-microRNA 724-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97132Tol2-lyzC-microRNA 727-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97133Tol2-lyzC-microRNA 730-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97134Tol2-lyzC-microRNA 1388-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97135Tol2-lyzC-microRNA 2184-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97136Tol2-lyzC-microRNA 130c-1-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97137Tol2-lyzC-microRNA 143-1-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97138Tol2-lyzC-microRNA 338-2-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97139Tol2-lyzC-microRNA 725-Dendra2 overexpression of microRNA and Dendra2 in Zebrafish
97140miR-722 plix4.03Lentiviral tet-inducible expression of miR-722and Dendra 2
97141miR-vector plix4.03For lentiviral tet-inducible expression of miRNA and Dendra2
97162miR-722 pCDNAluciferase assay
105433tol2-lyzC-miR-10c-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105434tol2-lyzC-miR-16a-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105435tol2-lyzC-miR-23a-2-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105436tol2-lyzC-miR-26b-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105437tol2-lyzC-miR-27b-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105438tol2-lyzC-miR-100-1-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105439tol2-lyzC-miR-132-2-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105440tol2-lyzC-miR-152-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105441tol2-lyzC-miR-181c-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105442tol2-lyzC-miR-182-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105443tol2-lyzC-miR-429a-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105444tol2-lyzC-miR-454a-dendra2overexpression of microRNA and Dendra2 in Zebrafish
105445tol2-lyzC-miR-734-dendra2overexpression of microRNA and Dendra2 in Zebrafish
121122dre-cdk2 3'UTR sicheck2 199 mutluciferase assay
121123dre-cdk2 3'UTR sicheck2luciferase assay
121124hsa-cdk2 3'UTR sicheck2 mut luciferase assay
121125hsa-cdk2 3'UTR sicheck2luciferase assay
121126hsa-miR 19b plix 4.03inducible expression in cells
121127hsa-miR 99 plix 4.03inducible expression in cells
121128hsa-miR-100 plix4.03inducible expression in cells
121129hsa-miR199 plix 4.03inducible expression in cells
121130vector plix 4.03inducible expression in cells
121131Tol2-lyzC-mcherry-2a-CDK2neutrophil specific expression
121132Tol2-lyzC-mcherry-2a-CDK2-D145Nneutrophil specific expression
121133hsa-miR-199-dendra2 PCDNAmammalian cell expression
121134vector-dendra2 PCDNAmammalian cell expression

Antibodies from Article